Investigating the Effect of Substituting a Single Cysteine Residue on the Thermal Stability of an Engineered Sweet Protein, Single-Chain Monellin

Kyosuke Ohnuma, Atsuko Yamashita, Norihisa Yasui

The Protein Journal　2023/09/22　Research paper (scientific journal)

Direct binding of calmodulin to the cytosolic C-terminal regions of sweet/umami taste receptors

Atsuki Yoshida, Ayumi Ito, Norihisa Yasui, Atsuko Yamashita

The Journal of Biochemistry　Vol. 174　No. 5　p. 451-459　2023/08/01　Research paper (scientific journal)

Structure and mechanism of oxalate transporter OxlT in an oxalate-degrading bacterium in the gut microbiota

Titouan Jaunet-Lahary, Tatsuro Shimamura, Masahiro Hayashi, Norimichi Nomura, Kouta Hirasawa, Tetsuya Shimizu, Masao Yamashita, Naotaka Tsutsumi, Yuta Suehiro, Keiichi Kojima, Yuki Sudo, Takashi Tamura, Hiroko Iwanari, Takao Hamakubo, So Iwata, Kei-ichi Okazaki, Teruhisa Hirai, Atsuko Yamashita

Nature Communications　Vol. 14　No. 1　2023/04/03　Research paper (scientific journal)

Chloride ions evoke taste sensations by binding to the extracellular ligand-binding domain of sweet/umami taste receptors

Nanako Atsumi, Keiko Yasumatsu, Yuriko Takashina, Chiaki Ito, Norihisa Yasui, Robert F Margolskee, Atsuko Yamashita

eLife　Vol. 12　2023/02/28　Research paper (scientific journal)

Pivotal role for S-nitrosylation of DNA methyltransferase 3B in epigenetic regulation of tumorigenesis.

Kosaku Okuda, Kengo Nakahara, Akihiro Ito, Yuta Iijima, Ryosuke Nomura, Ashutosh Kumar, Kana Fujikawa, Kazuya Adachi, Yuki Shimada, Satoshi Fujio, Reina Yamamoto, Nobumasa Takasugi, Kunishige Onuma, Mitsuhiko Osaki, Futoshi Okada, Taichi Ukegawa, Yasuo Takeuchi, Norihisa Yasui, Atsuko Yamashita, Hiroyuki Marusawa, Yosuke Matsushita, Toyomasa Katagiri, Takahiro Shibata, Koji Uchida, Sheng-Yong Niu, Nhi B Lang, Tomohiro Nakamura, Kam Y J Zhang, Stuart A Lipton, Takashi Uehara

Nature communications　Vol. 14　No. 1　p. 621-621　2023/02/04　Research paper (scientific journal)

Development of hidden Markov modeling method for molecular orientations and structure estimation from high-speed atomic force microscopy time-series images.

Tomonori Ogane, Daisuke Noshiro, Toshio Ando, Atsuko Yamashita, Yuji Sugita, Yasuhiro Matsunaga

PLoS computational biology　Vol. 18　No. 12　2022/12　Research paper (scientific journal)

T1R2-mediated sweet sensing in a lizard.

Liang Q, Ko MC, Ng NSR, Borja Reh, Lee JGH, Yamashita A, Nishihara H, Toda Y, Baldwin MW

Current biology : CB　Vol. 32　No. 23　p. R1302-R1303　2022/12/01　Research paper (scientific journal)

An optogenetic assay method for electrogenic transporters using Escherichia coli co‐expressing light‐driven proton pump

Masahiro Hayashi, Keiichi Kojima, Yuki Sudo, Atsuko Yamashita

Protein Science　Vol. 30　No. 10　p. 2161-2169　2021/07/10　Research paper (scientific journal)

A sweet protein monellin as a non-antibody scaffold for synthetic binding proteins

Norihisa Yasui, Kazuaki Nakamura, Atsuko Yamashita

The Journal of Biochemistry　Vol. 169　No. 5　p. 585-599　2021/07/03　Research paper (scientific journal)

Current pivotal strategies leading a difficult target protein to a sample suitable for crystallographic analysis

Atsuko Yamashita

Biochemical Society Transactions　Vol. 48　No. 4　p. 1661-1673　2020/08/28　Research paper (scientific journal)

Differential scanning fluorimetric analysis of the amino-acid binding to taste receptor using a model receptor protein, the ligand-binding domain of fish T1r2a/T1r3

Takashi Yoshida, Norihisa Yasui, Yuko Kusakabe, Chiaki Ito, Miki Akamatsu, Atsuko Yamashita

Plos One　Vol. 14　2019/06/13　Research paper (scientific journal)

Specific modification at the C-terminal lysine residue of the green fluorescent protein variant, GFPuv, expressed in Escherichia coli

Takahiro Nakatani, Norihisa Yasui, Issei Tamura, Atsuko Yamashita

Scientific Reports　Vol. 9　2019/03/18　Research paper (scientific journal)

うま味受容体細胞外リガンド結合ドメインのX線結晶構造解析

細谷 麻以子, 山下 敦子

SPring-8/SACLA利用研究成果集　Vol. 7　2019/01

Structure-function relationships of olfactory and taste receptors

Maik Behrens, Loïc Briand, Claire A. de March, Hiroaki Matsunami, Atsuko Yamashita, Wolfgang Meyerhof, Simone Weyand

Chemical Senses　Vol. 43　No. 2　p. 81-87　2018/02/02　Research paper (scientific journal)

A large-scale expression strategy for multimeric extracellular protein complexes using Drosophila S2 cells and its application to the recombinant expression of heterodimeric ligand-binding domains of taste receptor

Atsuko Yamashita, Eriko Nango, Yuji Ashikawa

PROTEIN SCIENCE　Vol. 26　No. 11　p. 2291-2301　2017/11　Research paper (scientific journal)

Structural basis for perception of diverse chemical substances by T1r taste receptors

Nipawan Nuemket, Norihisa Yasui, Yuko Kusakabe, Yukiyo Nomura, Nanako Atsumi, Shuji Akiyama, Eriko Nango, Yukinari Kato, Mika K. Kaneko, Junichi Takagi, Maiko Hosotani, Atsuko Yamashita

NATURE COMMUNICATIONS　Vol. 8　No. 1　2017/05　Research paper (scientific journal)

Taste substance binding elicits conformational change of taste receptor T1r heterodimer extracellular domains

Eriko Nango, Shuji Akiyama, Saori Maki-Yonekura, Yuji Ashikawa, Yuko Kusakabe, Elena Krayukhina, Takahiro Maruno, Susumu Uchiyama, Nipawan Nuemket, Koji Yonekura, Madoka Shimizu, Nanako Atsumi, Norihisa Yasui, Takaaki Hikima, Masaki Yamamoto, Yuji Kobayashi, Atsuko Yamashita

SCIENTIFIC REPORTS　Vol. 6　2016/05　Research paper (scientific journal)

Studies on an Acetylcholine Binding Protein Identify a Basic Residue in Loop G on the beta 1 Strand as a New Structural Determinant of Neonicotinoid Actions

Makoto Ihara, Toshihide Okajima, Atsuko Yamashita, Takuma Oda, Takuya Asano, Mikana Matsui, David B. Sattelle, Kazuhiko Matsuda

MOLECULAR PHARMACOLOGY　Vol. 86　No. 6　p. 736-746　2014/12　Research paper (scientific journal)

Distinct Human and Mouse Membrane Trafficking Systems for Sweet Taste Receptors T1r2 and T1r3

Madoka Shimizu, Masao Goto, Takayuki Kawai, Atsuko Yamashita, Yuko Kusakabe

PLOS ONE　Vol. 9　No. 7　2014/07　Research paper (scientific journal)

General flexible nature of the cytosolic regions of fungal transient receptor potential (TRP) channels, revealed by expression screening using GFP-fusion techniques

Makoto Ihara, Yoshitaka Takano, Atsuko Yamashita

PROTEIN SCIENCE　Vol. 23　No. 7　p. 923-931　2014/07　Research paper (scientific journal)

Molecular bases of multimodal regulation of a fungal transient receptor potential (TRP) channel

Makoto Ihara, Shin Hamamoto, Yohei Miyanoiri, Mitsuhiro Takeda, Masatsune Kainosho, Isamu Yabe, Nobuyuki Uozumi, Atsuko Yamashita

Journal of Biological Chemistry　Vol. 288　No. 21　p. 15303-15317　2013/05/24　Research paper (scientific journal)

GFP-based evaluation system of recombinant expression through the secretory pathway in insect cells and its application to the extracellular domains of class C GPCRs

Yuji Ashikawa, Makoto Ihara, Noriko Matsuura, Yuko Fukunaga, Yuko Kusakabe, Atsuko Yamashita

PROTEIN SCIENCE　Vol. 20　No. 10　p. 1720-1734　2011/10　Research paper (scientific journal)

High-resolution Native-PAGE for membrane proteins capable of fluorescence detection and hydrodynamic state evaluation

Makoto Ihara, Noriko Matsuura, Atsuko Yamashita

ANALYTICAL BIOCHEMISTRY　Vol. 412　No. 2　p. 217-223　2011/05　Research paper (scientific journal)

A Competitive Inhibitor Traps LeuT in an Open-to-Out Conformation

Satinder K. Singh, Chayne L. Piscitelli, Atsuko Yamashita, Eric Gouaux

SCIENCE　Vol. 322　No. 5908　p. 1655-1661　2008/12　Research paper (scientific journal)

Crystal structures of Lymnaea stagnalis AChBP in complex with neonicotinoid insecticides imidacloprid and clothianidin

Makoto Ihara, Toshihide Okajima, Atsuko Yamashita, Takuma Oda, Koichi Hirata, Hisashi Nishiwaki, Takako Morimoto, Miki Akamatsu, Yuji Ashikawa, Shun'Ichi Kuroda, Ryosuke Mega, Seiki Kuramitsu, David B. Sattelle, Kazuhiko Matsuda

Invertebrate Neuroscience　Vol. 8　No. 2　p. 71-81　2008/06　Research paper (scientific journal)

Antidepressant binding site in a bacterial homologue of neurotransmitter transporters

Satinder K. Singh, Atsuko Yamashita, Eric Gouaux

NATURE　Vol. 448　No. 7156　p. 952-956　2007/08　Research paper (scientific journal)

S3h1-2 Crystal structure of a bacterial homolog of Na^+/Cl^- -dependent neurotransmitter transporters(S3-h1: "Structural Aspects of Channel and Transporter Proteins",Symposia,Abstract,Meeting Program of EABS & BSJ 2006)

Yamashita Atsuko, Singh Satinder_K, Kawate Toshimitsu, Jin Yan, Gouaux Eric

Seibutsu Butsuri　Vol. 46　No. 2　2006

Structural basis of actin filament capping at the barbed-end: a cryo-electron microscopy study

Atsuko Yamashita

The EMBO Journal　Vol. 25　No. 23　2006　Research paper (scientific journal)

Crystal structure of a bacterial homologue of Na+/Cl--dependent neurotransmitter transporters

A Yamashita, SK Singh, T Kawate, Y Jin, E Gouaux

NATURE　Vol. 437　No. 7056　p. 215-223　2005/09　Research paper (scientific journal)

2P222 A new image analysis to determine three dimensional structures of ends of actin filaments from cryo electron micrographs

Narita A., Yamashita A., Maeda Y.

Seibutsu Butsuri　Vol. 45　2005

Capping protein binding to actin in yeast: biochemical mechanism and physiological relevance

Kim, K, Yamashita, A, Wear, MA, Maeda, Y, Cooper, JA

Journal of Cell Biology　Vol. 164　No. 4　p. 567-80　2004　Research paper (scientific journal)

Enzyme Structure Prior to Reaction Initiation Capturing with Laue Diffraction and its Reaction Mechanism : Tropinone Reductase-II-Substrate Complexes

YAMASHITA Atsuko, KATO Hiroaki

X-RAYS　Vol. 45　No. 6　p. 371-377　2003/12/25

Crystal structure of CapZ: structural basis for actin filament barbed end capping

A. Yamashita

The EMBO Journal　Vol. 22　No. 7　p. 1529-1538　2003/04/01　Research paper (scientific journal)

Structure of the core domain of human cardiac troponin in the Ca2+-saturated form

Atsuko Yamashita

Nature　Vol. 424　No. 6944　2003　Research paper (scientific journal)

How capping protein binds the barbed end of the actin filament

Wear, MA, Yamashita, A, Kim, K, Maeda, Y, Cooper, JA

Current Biology　Vol. 13　No. 17　p. 1531-7　2003　Research paper (scientific journal)

Capturing Enzyme Structure Prior to Reaction Initiation: Tropinone Reductase-II−Substrate Complexes ^‡

Atsuko Yamashita

Biochemistry　Vol. 42　No. 19　2003　Research paper (scientific journal)

2B1415 Crystal structure and actin-binding mechanism of actin filament capping protein CapZ

Yamashita A., Maeda K., Maeda Y.

Seibutsu Butsuri　Vol. 42　No. 2　2002

1E1500 Crystal structures of Troponin ternary complexes and insights into regulation of muscle contraction

Takeda S., Yamashita A., Maeda K., Maeda Y.

Seibutsu Butsuri　Vol. 42　No. 2　2002

Crystal structure of the C-terminal half of tropomodulin and structural basis of actin filament pointed-end capping

Krieger, I, Kostyukova, A, Yamashita, A, Nitanai, Y, Maeda, Y

Biophysical Journal　Vol. 83　No. 5　p. 2716-25　2002　Research paper (scientific journal)

Strategy for Crystallization and structural analysis of muscle regulatory protein complex

Takeda S., Yamashita A., Maeda Y.

Seibutsu Butsuri　Vol. 41　2001

Crystallographic structure analysis of actin capping protein Capz

Yamashita A., Maeda K., Maeda Y.

Seibutsu Butsuri　Vol. 41　2001

Crystallization and preliminary x-ray analysis of troponin ternary complex

Takeda S., Yamashita A., Maeda K., Kreiger Inna, Maeda Y.

Seibutsu Butsuri　Vol. 40　2000

Structure of Tropinone Reductase-II Complexed with NADP ⁺ and Pseudotropine at 1.9 Å Resolution: Implication for Stereospecific Substrate Binding and Catalysis ^‡

Atsuko Yamashita

Biochemistry　Vol. 38　No. 24　1999　Research paper (scientific journal)

Crystallization and preliminary crystallographic study of tropinone reductase II from Datura stramonium

Atsuko Yamashita

Acta Crystallographica Section D Structural Biology　Vol. 54　1998　Research paper (scientific journal)

Crystal structures of two tropinone reductases: Different reaction stereospecificities in the same protein fold

Nakajima, K, Yamashita, A, Akama, H, Nakatsu, T, Kato, H, Hashimoto, T, Oda, J, Yamada, Y

Proceedings of the National Academy of Sciences of the United States of America　Vol. 95　No. 9　p. 4876-4881　1998　Research paper (scientific journal)

Direct interaction between mitochondrial succinate-ubiquinone and ubiquinol-cytochrome c oxidoreductases probed by sensitivity to quinone-related inhibitors

Yamashita, A, Miyoshi, H, Hatano, T, Iwamura, H

Journal of Biochemistry　Vol. 120　No. 2　p. 377-384　1996　Research paper (scientific journal)